Spectral subtraction can be used, but it may damage signals out of the band of interest (outside of the hum frequencies - - - usually which are 60/180 Hz. I would use the Harmonic reject filter with the number of harmonics set to the minimum number to do the job. Often, I set it for 60 Hz and two odd harmonics and that does the trick for me (assuming it is ground loop induced hum).
Craig
Craig
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